CCR5 anticorps

N° du produit ABIN7835902

L’anticorps anti-CCR5 Monoclonal Rat est utilisé pour la détection de CCR5 dans des échantillons de Humain. Il a été validé pour FACS et IF.

Aperçu rapide pour CCR5 anticorps (ABIN7835902)

Antigène: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CCR5 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: HEK-1-85a

Détail du produit anti-CCR5 anticorps

Fonction: Rat monoclonal antibody to CD195

Specificité: Recognizes human CD195

Purification: The antibody was purified by affinity chromatography.

Immunogène: CHO cells transfected with human CCR5

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur CCR5

Antigène: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Autre désignation: CCR5

Sujet: CMKBR5, C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, HIV-1 fusion coreceptor, CD195

ID gène: 1234

UniProt: P51681

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size