CD209b Antigen (CD209B) anticorps

N° du produit ABIN7835948

Cet anticorps anti-CD209b Antigen Monoclonal Hamster arménien (Clone 22D1) (ABIN7835948) détecte spécifiquement CD209b Antigen dans FACS et IF. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour CD209b Antigen (CD209B) anticorps (ABIN7835948)

Antigène: CD209b Antigen (CD209B)

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 22D1

Détail du produit

Fonction: Armenian Hamster monoclonal antibody to CD209B

Specificité: Recognizes mouse CD209B

Purification: The antibody was purified by affinity chromatography.

Immunogène: Partial mouse CD209B recombinant protein (74-325 aa)

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détail du antigène

Antigène: CD209b Antigen (CD209B)

Autre désignation: CD209B

Sujet: CD209 antigen-like protein B, DC-SIGN-related protein 1, DC-SIGNR1, OtB7, CD209

ID gène: 69165