LAG3 anticorps

N° du produit ABIN7835970

Cet anticorps Rat Monoclonal détecte spécifiquement LAG3 dans FACS et IF. Il présente une réactivité avec des échantillons de Souris.

Aperçu rapide pour LAG3 anticorps (ABIN7835970)

Antigène: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp LAG3 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: C9B7W

Détail du produit anti-LAG3 anticorps

Fonction: Rat monoclonal antibody to CD223

Specificité: Recognizes mouse CD223

Purification: The antibody was purified by affinity chromatography.

Immunogène: Murine CD223-Ig fusion protein

Information d'application

Indications d'application: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-rat IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur LAG3

Antigène: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Autre désignation: LAG3

Sujet: FDC, Lymphocyte activation gene 3 protein, LAG-3, Protein FDC, CD223

ID gène: 16768

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints