CD235a/GYPA anticorps (PE)

N° du produit ABIN7835986

L’anticorps anti-CD235a/GYPA Monoclonal Souris est utilisé pour la détection de CD235a/GYPA dans des échantillons de Humain. Il a été validé pour FACS et IF.

Aperçu rapide pour CD235a/GYPA anticorps (PE) (ABIN7835986)

Antigène: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD235a/GYPA est conjugé à/à la PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: JC159

Détail du produit anti-CD235a/GYPA anticorps (PE)

Fonction: Mouse monoclonal antibody PE labled to CD235a

Specificité: Recognizes human CD235a

Purification: The antibody was purified by affinity chromatography.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur CD235a/GYPA

Antigène: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Autre désignation: GYPA

Sujet: GPA, Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha, CD235a

ID gène: 2993

UniProt: P02724

Pathways: Maintenance of Protein Location