CD276 anticorps

N° du produit ABIN7836050

Cet anticorps anti-CD276 Monoclonal Rat (Clone MJ18) (ABIN7836050) détecte spécifiquement CD276 dans FACS et IF. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour CD276 anticorps (ABIN7836050)

Antigène: CD276

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD276 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: MJ18

Détail du produit anti-CD276 anticorps

Fonction: Rat monoclonal antibody to CD276

Specificité: Recognizes mouse CD276

Purification: The antibody was purified by affinity chromatography.

Immunogène: Mouse B7-H3 transfected L cell and P815

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur CD276

Antigène: CD276

Autre désignation: CD276

Sujet: B7H3, CD276 antigen, 4Ig-B7-H3, B7 homolog 3, B7-H3, Costimulatory molecule, CD276

ID gène: 102657

Pathways: Cancer Immune Checkpoints