ICOS anticorps

N° du produit ABIN7836054

Cet anticorps Hamster arménien Monoclonal détecte spécifiquement ICOS dans FACS et IF. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour ICOS anticorps (ABIN7836054)

Antigène: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Reactivité: Humain, Souris, Rat

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp ICOS est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: C398-4A

Détail du produit anti-ICOS anticorps

Fonction: Armenian Hamster monoclonal antibody to CD278

Specificité: Recognizes human/mouse/rat CD278

Purification: The antibody was purified by affinity chromatography.

Immunogène: Mouse T cell clone D10.G4.1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur ICOS

Antigène: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Autre désignation: ICOS

Sujet: AILIM, Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, CD278

ID gène: 29851, 54167, 64545

UniProt: Q9Y6W8

Pathways: Cancer Immune Checkpoints