ICOS anticorps (APC)

N° du produit ABIN7836055

L’anticorps anti-ICOS Monoclonal Hamster arménien est utilisé pour la détection de ICOS dans des échantillons de Humain. Il a été validé pour FACS et IF.

Aperçu rapide pour ICOS anticorps (APC) (ABIN7836055)

Antigène: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Reactivité: Humain

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp ICOS est conjugé à/à la APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: C398-4A

Détail du produit anti-ICOS anticorps (APC)

Fonction: Armenian Hamster monoclonal antibody APC labled to CD278

Specificité: Recognizes human CD278

Purification: The antibody was purified by affinity chromatography.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur ICOS

Antigène: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Autre désignation: ICOS

Sujet: AILIM, Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, CD278

ID gène: 29851

UniProt: Q9Y6W8

Pathways: Cancer Immune Checkpoints