FCRL5 anticorps

N° du produit ABIN7836119

Cet anticorps anti-FCRL5 Monoclonal Souris (Clone 509f6) (ABIN7836119) détecte spécifiquement FCRL5 dans FACS et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour FCRL5 anticorps (ABIN7836119)

Antigène: FCRL5 (Fc Receptor-Like 5 (FCRL5))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FCRL5 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 509f6

Détail du produit anti-FCRL5 anticorps

Fonction: Mouse monoclonal antibody to CD307e

Specificité: Recognizes human CD307e

Purification: The antibody was purified by affinity chromatography.

Immunogène: P815 cells transfected with FcRL5

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur FCRL5

Antigène: FCRL5 (Fc Receptor-Like 5 (FCRL5))

Autre désignation: FCRL5

Sujet: FCRH5, IRTA2, Fc receptor-like protein 5, FcR-like protein 5, FcRL5, BXMAS1, Fc receptor homolog 5, FcRH5, Immune receptor translocation-associated protein 2, CD307e

ID gène: 83416

UniProt: Q96RD9