TIM3 anticorps (PE)

N° du produit ABIN7836219

Cet anticorps Souris Monoclonal détecte spécifiquement TIM3 dans FACS et IF. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour TIM3 anticorps (PE) (ABIN7836219)

Antigène: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TIM3 est conjugé à/à la PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: F38-2E2

Détail du produit anti-TIM3 anticorps (PE)

Fonction: Mouse monoclonal antibody PE labled to CD366

Specificité: Recognizes human CD366

Purification: The antibody was purified by affinity chromatography.

Immunogène: Human Tim-3 fusion protein

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur TIM3

Antigène: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Autre désignation: HAVCR2

Sujet: TIM3, TIMD3, Hepatitis A virus cellular receptor 2, HAVcr-2, T-cell immunoglobulin and mucin domain-containing protein 3, TIMD-3, T-cell immunoglobulin mucin receptor 3, TIM-3, T-cell membrane protein 3

ID gène: 84868

UniProt: Q8TDQ0

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints