CD43 anticorps (PE-Cy5)

N° du produit ABIN7836311

Cet anticorps anti-CD43 Monoclonal Souris conjugué à PE-Cy5 (Clone DF-T1) (ABIN7836311) détecte spécifiquement CD43 dans FACS et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CD43 anticorps (PE-Cy5) (ABIN7836311)

Antigène: CD43 (SPN) (Sialophorin (SPN))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD43 est conjugé à/à la PE-Cy5

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: DF-T1

Détail du produit anti-CD43 anticorps (PE-Cy5)

Fonction: Mouse monoclonal antibody PE/Cy5 labled to CD43

Specificité: Recognizes human CD43

Purification: The antibody was purified by affinity chromatography.

Immunogène: MV4-11 cell line

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur CD43

Antigène: CD43 (SPN) (Sialophorin (SPN))

Autre désignation: SPN

Sujet: CD43, Leukosialin, Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein, Sialophorin, CD43

ID gène: 6693

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity