CD51 anticorps (FITC)

N° du produit ABIN7836446

Cet anticorps anti-CD51 Monoclonal Souris conjugué à FITC (Clone 13C2) (ABIN7836446) détecte spécifiquement CD51 dans FACS et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CD51 anticorps (FITC) (ABIN7836446)

Antigène: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD51 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 13C2

Détail du produit anti-CD51 anticorps (FITC)

Fonction: Mouse monoclonal antibody FITC labled to CD51

Specificité: Recognizes human CD51

Purification: The antibody was purified by affinity chromatography.

Immunogène: Cell suspension containing osteoclasts from osteoclastomas

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur CD51

Antigène: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Autre désignation: ITGAV

Sujet: MSK8, VNRA, Integrin alpha-V, Vitronectin receptor subunit alpha, CD51

ID gène: 3685

UniProt: P06756

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Integrin Complex