CD53 anticorps (FITC)

N° du produit ABIN7836450

Cet anticorps Souris Monoclonal détecte spécifiquement CD53 dans FACS et IF. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour CD53 anticorps (FITC) (ABIN7836450)

Antigène: CD53

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD53 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: C53-2

Détail du produit anti-CD53 anticorps (FITC)

Fonction: Mouse monoclonal antibody FITC labled to CD53

Specificité: Recognizes human CD53

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD53.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur CD53

Antigène: CD53

Autre désignation: CD53

Sujet: MOX44, TSPAN25, Leukocyte surface antigen CD53, Cell surface glycoprotein CD53, Tetraspanin-25, Tspan-25, CD antigen CD53

ID gène: 963

UniProt: P19397