CD58 anticorps (FITC)

N° du produit ABIN7836490

L’anticorps anti-CD58 Monoclonal Souris est utilisé pour la détection de CD58 dans des échantillons de Humain. Il a été validé pour FACS et IF.

Aperçu rapide pour CD58 anticorps (FITC) (ABIN7836490)

Antigène: CD58

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD58 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: TS2-9

Détail du produit anti-CD58 anticorps (FITC)

Fonction: Mouse monoclonal antibody FITC labled to CD58

Specificité: Recognizes human CD58

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD58.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur CD58

Antigène: CD58

Autre désignation: CD58

Sujet: LFA3, Lymphocyte function-associated antigen 3, Ag3, Surface glycoprotein LFA-3, CD antigen CD58

ID gène: 965

UniProt: P19256