CD83 anticorps (PE)

N° du produit ABIN7836632

Cet anticorps anti-CD83 Monoclonal Souris conjugué à PE (Clone HB15e) (ABIN7836632) détecte spécifiquement CD83 dans FACS et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CD83 anticorps (PE) (ABIN7836632)

Antigène: CD83

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD83 est conjugé à/à la PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: HB15e

Détail du produit anti-CD83 anticorps (PE)

Fonction: Mouse monoclonal antibody PE labled to CD83

Specificité: Recognizes human CD83

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD83.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur CD83

Antigène: CD83

Autre désignation: CD83

Sujet: CD83 antigen, hCD83, B-cell activation protein, Cell surface protein HB15, CD antigen CD83

ID gène: 9308

UniProt: Q01151