CD9 anticorps (Biotin)

N° du produit ABIN7836672

Cet anticorps Souris Monoclonal détecte spécifiquement CD9 dans FACS et IF. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour CD9 anticorps (Biotin) (ABIN7836672)

Antigène: CD9

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD9 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: HI9a

Détail du produit anti-CD9 anticorps (Biotin)

Fonction: Mouse monoclonal antibody Biotin labled to CD9

Specificité: Recognizes human CD9

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD9.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature. 7. Repeat step 5. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur CD9

Antigène: CD9

Autre désignation: CD9

Sujet: MIC3, TSPAN29, CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, MRP-1, Tetraspanin-29, Tspan-29, p24, CD antigen CD9

ID gène: 928

UniProt: P21926

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization