CXCL9 anticorps

N° du produit ABIN7837366

L’anticorps anti-CXCL9 Monoclonal Hamster arménien est utilisé pour la détection de CXCL9 dans des échantillons de Souris. Il a été validé pour FACS et IF.

Aperçu rapide pour CXCL9 anticorps (ABIN7837366)

Antigène: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp CXCL9 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: MIG-2F5-5

Détail du produit anti-CXCL9 anticorps

Fonction: Armenian Hamster monoclonal antibody to CXCL9

Specificité: Recognizes mouse CXCL9

Purification: The antibody was purified by affinity chromatography.

Immunogène: Mouse CXCL9 Recombinant Protein

Information d'application

Indications d'application: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur CXCL9

Antigène: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Autre désignation: CXCL9

Sujet: CMK, MIG, SCYB9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, HuMIG, MIG, Small-inducible cytokine B9

ID gène: 17329