Hepsin anticorps

N° du produit ABIN7839442

L’anticorps anti-Hepsin Monoclonal Souris est utilisé pour la détection de Hepsin dans des échantillons de Humain. Il a été validé pour IF et FACS.

Aperçu rapide pour Hepsin anticorps (ABIN7839442)

Antigène: Hepsin (HPN)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Hepsin est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 2D5

Détail du produit anti-Hepsin anticorps

Fonction: Mouse monoclonal antibody to Hepsin

Specificité: Recognizes human Hepsin

Purification: The antibody was purified by affinity chromatography.

Immunogène: Recombinant fusion protein of human HPN

Information d'application

Indications d'application: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur Hepsin

Antigène: Hepsin (HPN)

Autre désignation: HPN

Sujet: TMPRSS1, Serine protease hepsin, Transmembrane protease serine 1

ID gène: 3249

UniProt: P05981

Pathways: Regulation of Hormone Metabolic Process, Sensory Perception of Sound