Ki-67 anticorps (FITC)

N° du produit ABIN7840347

Cet anticorps Souris Monoclonal détecte spécifiquement Ki-67 dans FACS et IF. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Ki-67 anticorps (FITC) (ABIN7840347)

Antigène: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Ki-67 est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 3D5

Détail du produit anti-Ki-67 anticorps (FITC)

Fonction: Mouse monoclonal antibody FITC labled to Ki67

Specificité: Recognizes human Ki67

Purification: The antibody was purified by affinity chromatography.

Immunogène: Recombinant fusion protein of human Ki67.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur Ki-67

Antigène: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Autre désignation: MKI67

Sujet: Antigen KI-67

ID gène: 4288

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process