LY6 anticorps (Pacific Blue)

N° du produit ABIN7840915

L’anticorps anti-LY6 Monoclonal Rat est utilisé pour la détection de LY6 dans des échantillons de Souris. Il a été validé pour FACS et IF.

Aperçu rapide pour LY6 anticorps (Pacific Blue) (ABIN7840915)

Antigène: LY6 (Lymphocyte Antigen 6 Complex (LY6))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp LY6 est conjugé à/à la Pacific Blue

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: D7

Détail du produit anti-LY6 anticorps (Pacific Blue)

Fonction: Rat monoclonal antibody Pacific Blue labled to Ly-6A/E

Specificité: Recognizes mouse Ly-6A/E

Purification: The antibody was purified by affinity chromatography.

Immunogène: IL-2 dependent mouse (C57BL/6) cytotoxic T cell line CTL-L

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur LY6

Antigène: LY6 (Lymphocyte Antigen 6 Complex (LY6))

Autre désignation: Ly6

Sujet: Ly6, Lymphocyte antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Stem cell antigen 1, SCA-1, T-cell-activating protein, TAP, Ly67, Sca-2, Tsa-1, Lymphocyte antigen 6E, Ly-6E, Stem cell antigen 2, Thymic shared antigen 1, TSA-1

ID gène: 110454