H2-D1 anticorps

N° du produit ABIN7841254

L’anticorps anti-H2-D1 Monoclonal Souris est utilisé pour la détection de H2-D1 dans des échantillons de Souris. Il a été validé pour FACS et IF.

Aperçu rapide pour H2-D1 anticorps (ABIN7841254)

Antigène: H2-D1 (Histocompatibility 2, D Region Locus 1 (H2-D1))

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp H2-D1 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: B22-249

Détail du produit anti-H2-D1 anticorps

Fonction: Mouse monoclonal antibody to MHC Class I (H-2Db)

Specificité: Recognizes mouse MHC Class I (H-2Db)

Purification: The antibody was purified by affinity chromatography.

Immunogène: Mouse H-2Db

Information d'application

Indications d'application: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur H2-D1

Antigène: H2-D1 (Histocompatibility 2, D Region Locus 1 (H2-D1))

Autre désignation: H2-D1

Sujet: H-2D(B), H-2 class I histocompatibility antigen D-B alpha chain

ID gène: 14964

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process