HIST1H2AA anticorps (PE)

N° du produit ABIN7841260

L’anticorps anti-HIST1H2AA Monoclonal Rat est utilisé pour la détection de HIST1H2AA dans des échantillons de Souris. Il a été validé pour IF et FACS.

Aperçu rapide pour HIST1H2AA anticorps (PE) (ABIN7841260)

Antigène: HIST1H2AA (Histone Cluster 1, H2aa (HIST1H2AA))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp HIST1H2AA est conjugé à/à la PE

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: NIMR-4

Détail du produit anti-HIST1H2AA anticorps (PE)

Fonction: Rat monoclonal antibody PE labled to MHC Class II

Specificité: Recognizes mouse MHC Class II

Purification: The antibody was purified by affinity chromatography.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur HIST1H2AA

Antigène: HIST1H2AA (Histone Cluster 1, H2aa (HIST1H2AA))

Autre désignation: H2-Aa

Sujet: Ia1, H2Aa, Ia-1, H-2Aa, Aalpha, IAalpha, I-Aalpha

ID gène: 14960