TRAC anticorps (FITC)

N° du produit ABIN7845263

Cet anticorps anti-TRAC Monoclonal Souris conjugué à FITC (Clone IP26) (ABIN7845263) détecte spécifiquement TRAC dans FACS et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour TRAC anticorps (FITC) (ABIN7845263)

Antigène: TRAC (T Cell Receptor alpha Constant (TRAC))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TRAC est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: IP26

Détail du produit anti-TRAC anticorps (FITC)

Fonction: Mouse monoclonal antibody FITC labled to TCR alpha/beta

Specificité: Recognizes human TCR alpha/beta

Purification: The antibody was purified by affinity chromatography.

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur TRAC

Antigène: TRAC (T Cell Receptor alpha Constant (TRAC))

Autre désignation: TRAC

Sujet: TCRA, T cell receptor alpha chain constant, T cell receptor beta constant 1

ID gène: 28755

UniProt: P01848