TCRb anticorps (APC)

N° du produit ABIN7845265

L’anticorps anti-TCRb Monoclonal Hamster arménien est utilisé pour la détection de TCRb dans des échantillons de Souris. Il a été validé pour FACS et IF.

Aperçu rapide pour TCRb anticorps (APC) (ABIN7845265)

Antigène: TCRb (T Cell Receptor beta Chain (TCRb))

Reactivité: Souris

Hôte: Hamster arménien

Clonalité: Monoclonal

Conjugué: Cet anticorp TCRb est conjugé à/à la APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: H57-597

Détail du produit anti-TCRb anticorps (APC)

Fonction: Armenian hamster monoclonal antibody APC labled to TCR beta

Specificité: Recognizes mouse TCR beta

Purification: The antibody was purified by affinity chromatography.

Immunogène: Affinity purified TCR from mouse DO-11.10 cells

Information d'application

Indications d'application: Flow: 10 μL / assay

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Shipped and store at 4°C for one year. Do not freeze.

Détails sur TCRb

Antigène: TCRb (T Cell Receptor beta Chain (TCRb))

Autre désignation: TCRB

Sujet: Tib, TCRbeta

ID gène: 21577