TCR alpha anticorps

N° du produit ABIN7845274

L’anticorps anti-TCR alpha Monoclonal Souris est utilisé pour la détection de TCR alpha dans des échantillons de Humain. Il a été validé pour FACS et IF.

Aperçu rapide pour TCR alpha anticorps (ABIN7845274)

Antigène: TCR alpha (TRA) (T Cell Receptor alpha Locus (TRA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TCR alpha est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 6B11

Détail du produit anti-TCR alpha anticorps

Fonction: Mouse monoclonal antibody to TCR V alpha 24-J alpha 18

Specificité: Recognizes human TCR V alpha 24-J alpha 18

Purification: The antibody was purified by affinity chromatography.

Immunogène: Peptide epitope in the CDR3 region of V alpha 24

Information d'application

Indications d'application: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Détails sur TCR alpha

Antigène: TCR alpha (TRA) (T Cell Receptor alpha Locus (TRA))

Autre désignation: TRA

Sujet: INKT cell, iNKT, invariant natural killer T cel, invariant NK T cell, invariant NKT cell

ID gène: 6955