Recombinant Vimentin anticorps (AA 2-466)

N° du produit ABIN7863496

Cet anticorps anti-Vimentin Monoclonal Souris (Clone rVIM-6431) (ABIN7863496) détecte spécifiquement Vimentin dans WB et IHC. L’anticorps est réactif avec des échantillons de Humain, Rat, Porc, Boeuf (Vache), Poulet, Chien, Cat et Cheval.

Aperçu rapide pour Recombinant Vimentin anticorps (AA 2-466) (ABIN7863496)

Antigène: Vimentin (VIM)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat, Porc, Boeuf (Vache), Poulet, Chien, Cat, Cheval

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RVIM-6431

Détail du produit anti-Vimentin anticorps

Épitope: AA 2-466

Expression System: HEK-293 Cells

Fonction: Recombinant Vimentin (Mesenchymal Cell Marker) Antibody

Specificité: This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Purification: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogène: A recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: Vimentin

Sujet:

VIM

Raji, U-87, Jurkat or HeLa cells. Human lymph node or tonsil. Human Heart, Human Skeletal muscle, Human Skin, A549.

Poids moléculaire: 57-60kDa

ID gène: 7431, 455493

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis