SPARC anticorps (AA 1-200)

N° du produit ABIN7863419

Cet anticorps Souris Monoclonal détecte spécifiquement SPARC dans WB et IHC. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour SPARC anticorps (AA 1-200) (ABIN7863419)

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SPARC est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: OSTN-3933

Détail du produit anti-SPARC anticorps

Épitope: AA 1-200

Fonction: SPARC / Osteonectin Antibody

Specificité: SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment (around aa1-200) of human SPARC protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide store at 2 to 8 °C. Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Date de péremption: 24 months

Détails sur SPARC

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Autre désignation: SPARC

Sujet:

Basement membrane protein 40, BM40, Cysteine rich protein, OI17, ON, Osteonectin, Secreted acidic cystein rich glycoprotein, Secreted protein acidic and cysteine rich, Secreted protein acidic and rich in cysteine (SPARC)

Human placenta, lymph node or spleen. JEG3 or A549 or A-375 cell lysates.

Poids moléculaire: 38kDa

ID gène: 6678, 111779, 708558

UniProt: P09486

Pathways: Autophagy