The extrinsic pathway of blood coagulation is initiated by contact of plasma factor VII with tissue factor, a cellular membrane glycoprotein that normally is segregated from the bloodstream but can be exposed after tissue injury or newly synthesized in endothelial cells or leukocytes after stimulation by endotoxin and cytokines. Inhibition of Factor VIIa tissue factor activity requires a plasma component (tissue factor pathway inhibitor (TFPI), lipoprotein-associated coagulation inhibitor (LACI) or extrinsic pathway inhibitor (EPI)) and factor Xa. TFPI directly inhibits factor Xa, and, in an Xa-dependent fashion, also inhibits the Factor VIIa tissue factor catalytic complex. TFPI is a multivalent, Kunitz-type proteinase inhibitor that circulates in association with plasma lipoproteins VLDL, LDL, and HDL. TFPI-2 (also known as placental protein 5) is a related glycoprotein that was originally isolated from human placenta.
Purification
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
Immunogène
Recombinant fragment (around aa100-235) of human TFPI2 protein (exact sequence is proprietary)
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
Prepared in 10 mM PBS
Agent conservateur
Azide free
Stock
-20 °C,-80 °C
Stockage commentaire
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.