Nanog anticorps (AA 100-250)

N° du produit ABIN7865247

Cet anticorps anti-Nanog Monoclonal Souris (Clone NANOG-9704) (ABIN7865247) détecte spécifiquement Nanog dans IHC. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Nanog anticorps (AA 100-250) (ABIN7865247)

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nanog est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: NANOG-9704

Détail du produit anti-Nanog anticorps

Épitope: AA 100-250

Fonction: Homeobox protein NANOG (Cancer Stem Cell Marker) Antibody

Specificité: The homeobox protein Nanog is a transcription factor crucial for maintaining the pluripotency of embryonic stem cells (ESCs) by inhibiting cell differentiation factors. In humans, the NANOG gene encodes this protein. Nanog operates alongside other factors such as Oct-4 and SOX2 to define ESC identity. It is also highly expressed in cancer stem cells, suggesting a potential role as an oncogene in promoting cancer development. Elevated levels of Nanog are linked to poor prognosis in cancer patients. Nanog shows strong and specific expression in carcinoma in situ (CIS), embryonal carcinomas, and seminomas but are absent in teratomas and yolk sac tumors. Research indicates that human embryonic stem cell-related genes, including OCT4, NANOG, STELLAR, and GDF3, are expressed in seminomas and breast carcinomas. Nanog positivity is notably associated with high-grade ovarian serous carcinoma but is not observed in benign, borderline, or low-grade serous lesions. One study highlights the cellular shuttling of Nanog and its increased stromal presence during cervical cancer progression. Furthermore, Nanog overexpression has been linked to factors such as tumor differentiation, lymph node metastasis, and tumor size, with studies suggesting its predictive value for reduced overall survival (OS) and disease-free survival (DFS) in lung cancer.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogène: Recombinant fragment (around aa100-250) of human NANOG protein (exact sequence is proprietary)

Isotype: IgG2b, lambda

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Détails sur Nanog

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Autre désignation: Homeobox protein NANOG

Sujet:

homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48

Human testicular carcinoma.

Poids moléculaire: 40kDa

ID gène: 79923

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance