CD45RA anticorps

N° du produit ABIN7865593

Cet anticorps anti-CD45RA Monoclonal Souris (Clone 158-4D3) (ABIN7865593) détecte spécifiquement CD45RA dans FACS, IF, IHC et ICC. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CD45RA anticorps (ABIN7865593)

Antigène: CD45RA

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD45RA est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: 158-4D3

Détail du produit anti-CD45RA anticorps

Fonction: anti-CD45RA mouse monoclonal, 158-4D3, purified

Purification: affinity chromatography

Immunogène: stimulated human leucocytes

Isotype: IgG2a kappa

Information d'application

Indications d'application:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: store at 2 - 8 °C

Détails sur CD45RA

Antigène: CD45RA

Autre désignation: CD45RA

Sujet: CD45 glycoprotein have various molecular weights on various cell types: B-cell 240 kDa, thymocytes 180 kDa, T-cell multiple bands. Reduced in PAGE gels: 180 and 240 kDa. Relevant epitopes are termed CD45RA (exon 4), CD45 RB (exon 5, CD45RC (exon 6) and CD45R or CD45RO (exons 4-6 spliced out). 158-4D3 was clustered in Boston at the Vth International CD workshop as anti CD45RA, useful to differentiate "naive" T-cells from activated (CD45R0+ve) T-cells, or to differentiate T-cell lymphomas (CD45R0+ve) from B-cell lymphomas (CD45RA+ve). Positive control: Daudi, Raji, IM9 and human lymphocytes. Human lymph node and tonsil.