Histone H1 anticorps
Aperçu rapide pour Histone H1 anticorps (ABIN7865649)
Antigène
Voir toutes Histone H1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Fonction
- anti-Histone H1 mouse monoclonal, AE-4, purified
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Purification
- affinity chromatography
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Immunogène
- human leukemia biopsy cells
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Isotype
- IgG2a kappa
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Indications d'application
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ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)
FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 100 μg/mL
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Buffer
- PBS with 0.02 % sodium azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Stockage commentaire
- store at 2 - 8 °C
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- Histone H1
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Sujet
- AE-4 recognizes histone H1 in the nuclei of animal and plant cells. It produces a speckled nuclear staining pattern in normal and malignant cells. Positive control: HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma or tonsil.
Antigène
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