ICAM-3/CD50 anticorps
Aperçu rapide pour ICAM-3/CD50 anticorps (ABIN7865666)
Antigène
Voir toutes ICAM-3/CD50 (ICAM3) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Fonction
- anti-CD50 mouse monoclonal, 189-2G9, purified
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Purification
- affinity chromatography
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Immunogène
- stimulated human leucocytes
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Isotype
- IgG2b kappa
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Indications d'application
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ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)
FACS_dilution: 1-2 μg/million cells
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 100 μg/mL
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Buffer
- PBS with 0.02 % sodium azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Stockage commentaire
- store at 2 - 8 °C
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- ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
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Autre désignation
- CD50
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Sujet
- 189-2G9 reacts with the cellular adhesion molecule CD50 (ICAM-3), a single chain polypeptide with a MW of 120 kDa. The protein is heavily glycosylated and resistant to phosphatidylinositol phospholipase C treatment so probably not PI-anchored. 186-2G9 has been clustered at the VIth GLDA International Workshop. 186-2D9 recognized the D1 domain of human CD50 and blocks binding of CD11a (α L-integrin) to CD50. Positive control: HL-60 or THP-1 cells. Lymph node and tonsil.
Antigène
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