ASRGL1 anticorps
Aperçu rapide pour ASRGL1 anticorps (ABIN7865675)
Antigène
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Fonction
- anti-CRASH mouse monoclonal, 7B6, purified
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Purification
- affinity chromatography
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Immunogène
- with CRASH recombinant protein and synthetic peptide
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Isotype
- IgG1 kappa
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Indications d'application
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IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
WB_dilution: 1:50-1:100 (1-2 μg/mL)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
elisa_dilution: assay dependent
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 100 μg/mL
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Buffer
- PBS with 0.02 % sodium azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Stockage commentaire
- store at 2 - 8 °C
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- ASRGL1 (Isoaspartyl Peptidase/L-Asparaginase (ASRGL1))
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Autre désignation
- CRASH
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Sujet
- 7B6 reacts with CRASH, a 308 AA glycoprotein sharing 32 % homology with human asparaginase and identical to Asparaginase-Like Protein (ALP) found in rat sperm. CRASH is found in a variety of tumors but only occasionally in normal tissues. Especially ovarian cancers are highly positive. CRASH is further found in brain tumors, some colonic cancers, some lung cancers, some prostate cancers, uterine cancers, some breast cancers and some thyroid cancers. It was not found in serum. In Western blot 3 bands are demonstrated of 45 kDa, 28 kDa and 15 kDa. Positive control: Ovarian cancer, KM22 breast cancer cells.
Antigène
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