KRT14 anticorps (C-Term)
Aperçu rapide pour KRT14 anticorps (C-Term) (ABIN7865678)
Antigène
Voir toutes KRT14 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- AA 459-472, C-Term
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Fonction
- anti-Keratin 14 mouse monoclonal, EBS-IF-004, purified
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Purification
- affinity chromatography
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Immunogène
- AA 459-472 at the carboxyterminal end of human cytokeratin 14, conjugated to KLH
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Isotype
- IgM kappa
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Indications d'application
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ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)
FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C
IHC-paraffin_dilution: 1:25-1:50 (2-4 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)
WB_dilution: 1:50-1:100 (1-2 μg/mL)
IHC-frozen_dilution: 1:25-1:50 (2-4 μg/mL)
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 100 μg/mL
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Buffer
- PBS with 0.02 % sodium azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Stockage commentaire
- store at 2 - 8 °C
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- KRT14 (Keratin 14 (KRT14))
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Autre désignation
- Keratin 14
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Sujet
- EBS-IF-004 reacts specifically with keratin 14 (50 kDa) in immunoblotting. In tissue sections EBS-IF-004 is positive with basal cells of non-keratinizing stratified epithelia, basal cells and suprabasal cells of the epidermis and gingiva, myoepithelial cells and squamous cell carcinomas. Positive control: Skin.
Antigène
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