KRT4 anticorps

N° du produit ABIN7865679

L’anticorps anti-KRT4 Monoclonal Souris est utilisé pour la détection de KRT4 dans des échantillons de Humain, Rat, Souris, Chien, Cat, Lapin, Chévre, Cobaye, Singe, Poulet, Furet et Xenopus laevis. Il a été validé pour WB, IHC, IF, FACS et ICC.

Aperçu rapide pour KRT4 anticorps (ABIN7865679)

Antigène: KRT4 (Keratin 4 (KRT4))

Reactivité: Humain, Rat, Souris, Chien, Cat, Lapin, Chévre, Cobaye, Singe, Poulet, Furet, Xenopus laevis

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KRT4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: C-11

Détail du produit anti-KRT4 anticorps

Fonction: anti-Keratin 4,5,6,8,10,13,18 mouse monoclonal, C-11, purified

Purification: affinity chromatography

Immunogène: cytoskeleton preparation of human A431 carcinoma cell line

Isotype: IgG1 kappa

Information d'application

Indications d'application:

ICC/IF_dilution: 1:100-1:200 (0.5-1.0 μg/mL)

FACS_dilution: 0.5-1.0 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 or digestion with trypsin at 1 mg/mL PBS recommended)

WB_dilution: 1:100 (1 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: store at 2 - 8 °C

Détails sur KRT4

Antigène: KRT4 (Keratin 4 (KRT4))

Autre désignation: Keratin 4

Sujet: C11 reacts with keratins : 4,5,6,8,10,13 and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Positive control: A431 cells, skin, lung.