Keratin 5/14 anticorps

N° du produit ABIN7865680

Cet anticorps anti-Keratin 5/14 Monoclonal Souris (Clone EBS-IF-001) (ABIN7865680) détecte spécifiquement Keratin 5/14 dans IHC, ICC, IF, FACS et WB. L’anticorps est réactif avec des échantillons de Humain et Cat.

Aperçu rapide pour Keratin 5/14 anticorps (ABIN7865680)

Antigène: Keratin 5/14 (KRT5/14)

Reactivité: Humain, Cat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Keratin 5/14 est non-conjugé

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB)

Clone: EBS-IF-001

Détail du produit anti-Keratin 5/14 anticorps

Fonction: anti-Keratin 5+14 mouse monoclonal, EBS-IF-001, purified

Purification: affinity chromatography

Immunogène: cytokeratin-enriched extract of feline tongue epithelium

Isotype: IgG2a kappa

Information d'application

Indications d'application:

ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)

FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C

WB_dilution: assay dependent

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: store at 2 - 8 °C

Détails sur Keratin 5/14

Antigène: Keratin 5/14 (KRT5/14)

Autre désignation: Keratin 5+14

Sujet: EBS-IF-001 Does not react with keratin polypeptides in immunoblotting. The specificity for keratin was established on the basis of antibody reactivity with intracytoplasmic intermediate filaments in epithelial, vimentin negative, human and feline cells. Distribution of the epitope strongly suggests that EBS-IF-001 reacts with keratins 5 and 14, most probably with a heterotypic complex formed by these keratins. EBS-IF-001 reacts positively with basal cells of all stratified epithelia, with myoepithelial cells and with most squamous cell carcinomas. It is useful in immunohistochemistry for typing of basal cells. Positive control: Tongue.