Keratin 7/17 anticorps
Aperçu rapide pour Keratin 7/17 anticorps (ABIN7865681)
Antigène
Voir toutes Keratin 7/17 (KRT7/17) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Fonction
- anti-Keratin 7+17 mouse monoclonal, C-46, purified
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Réactivité croisée (Details)
- not with ferret, mouse, rabbit, rat
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Purification
- affinity chromatography
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Immunogène
- purified human keratin 7, isolated from HeLa cytoskeleton
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Isotype
- IgG1 kappa
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Indications d'application
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ICC/IF_dilution: 1:50-1:100 (1-2 μg/mL)
FACS_dilution: 1-2 μg/million cells in 0,1 ml, fix cells in 4 % PFA for 10 min, at 4 °C, permeabilize with 0,2 % saponin or digitonin for 15 min, at 4 °C
IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment and/ or pepsin digestion recommended)
WB_dilution: 1:50-1:100 (1-2 μg/mL)
IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 100 μg/mL
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Buffer
- PBS with 0.02 % sodium azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Stockage commentaire
- store at 2 - 8 °C
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- Keratin 7/17 (KRT7/17)
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Autre désignation
- Keratin 7+17
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Sujet
- C-46 reacts with keratins 7 (54 kDa) and 17 (46 kDa). On formalin-fixed paraffin embedded sections C46 reacts with keratin 7 only. Strongly positive on most simple epithelia except for stomach, small intestine and colon mucosa, hepatocytes, pancreatic acini, renal tubules, also positive on some non-cornifying epithelia. Positive control: Relevant simple epithelia like ovary, lung, and breast.
Antigène
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