Proliferation Marker anticorps

N° du produit ABIN7865722

Cet anticorps anti-Proliferation Marker Monoclonal Souris (Clone IPO-38) (ABIN7865722) détecte spécifiquement Proliferation Marker dans IHC, WB, ELISA et IP. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Proliferation Marker anticorps (ABIN7865722)

Antigène: Proliferation Marker

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: IPO-38

Détail du produit

Fonction: anti-Proliferation Marker mouse monoclonal, IPO-38, purified

Purification: affinity chromatography

Immunogène: spleen cells of a patient with hairy cell leukemia

Isotype: IgM kappa

Information d'application

Indications d'application:

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: store at 2 - 8 °C

Détail du antigène

Antigène: Proliferation Marker

Sujet: IPO-38 reacts with a 12-14 kDa protein, as found in Western blots of Raji cells, and appears in the mitotic cycle earlier than Ki-67. Lymphocytes, induced to early G1 phase by 12h exposure to PHA, will become positive while non-stimulated lymphocytes remain negative. Mononuclear cells and granulocytes of healthy donors are negative, while various forms of leukemia and lymphoma including Hodgkin’s disease are positive for IPO-38, as are many solid tumors such as some breast, gastric and colonic cancers for which it may serve as tumor progression marker. Positive control: Raji cells.