CD2 anticorps

N° du produit ABIN7866122

L’anticorps anti-CD2 Monoclonal Souris est utilisé pour la détection de CD2 dans des échantillons de Humain. Il a été validé pour FACS.

Aperçu rapide pour CD2 anticorps (ABIN7866122)

Antigène: CD2

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD2 est non-conjugé

Application: Flow Cytometry (FACS)

Clone: T6-3

Détail du produit anti-CD2 anticorps

Fonction: Mouse anti Human CD2

Attributs du produit: Mouse anti human CD2 can be used for identification of human T cells and a subset of NK cells associated with the receptor for sheep erythocytes rosettes expressing the 45-50,000 M.W. surface antigen.

Purification: Protein A/G Chromatography

Immunogène: Derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with T Lymphocytes activated by a mixed lymphocyte culture.

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry: PBMC: Add 10 μL of antibody to 1 million PBMCs in 100 μL PBS. Mix gently and incubate for 15 minutes at 2 to 8oC. Wash twice with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 μL of antibody to 100 μL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse the whole blood. Wash once with PBS, incubate with an appropriate concentratio with a fluorochrome conjugated secondary antibody, wash again and analyze or fix with 0.5 % v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer's instructions for immunofluorescence analysis with a flow cytometer or microscope.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur CD2

Antigène: CD2

Autre désignation: CD2

Sujet: T-cell surface antigen CD2, CD2 cytoplasmic domain-binding protein

UniProt: P06729