TCOF1 anticorps (AA 1-1340)

N° du produit ABIN7868120

Cet anticorps Lapin Polyclonal détecte spécifiquement TCOF1 dans WB, ELISA, IF, ICC et FACS. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour TCOF1 anticorps (AA 1-1340) (ABIN7868120)

Antigène: TCOF1 (Treacher Collins-Franceschetti Syndrome 1 (TCOF1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TCOF1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-TCOF1 anticorps

Épitope: AA 1-1340

Fonction: TCOF1 Antibody / Treacle protein

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human TCOF1 recombinant protein (Position: M1-K1340) was used as the immunogen for the TCOF1 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the TCOF1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the TCOF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur TCOF1

Antigène: TCOF1 (Treacher Collins-Franceschetti Syndrome 1 (TCOF1))

Autre désignation: TCOF1

Sujet: TCOF1 antibody detects Treacle protein, a nucleolar phosphoprotein involved in ribosome biogenesis and craniofacial development. The UniProt recommended name is Treacle protein (TCOF1). This protein plays a key role in rRNA transcription, processing, and ribosomal subunit assembly, ensuring proper neural crest cell proliferation during embryogenesis.

Functionally, TCOF1 antibody identifies a 1,488-amino-acid protein localized to the nucleolus, where it interacts with upstream binding factor (UBF) and RNA polymerase I machinery. TCOF1 facilitates 47S pre-rRNA transcription and processing, linking nucleolar activity to cell growth and differentiation. It also participates in DNA damage response by stabilizing nucleolar integrity under stress.

The TCOF1 gene is located on chromosome 5q32-q33.1 and is highly expressed in neural crest-derived and proliferative tissues. Proper TCOF1 function is essential for craniofacial bone and cartilage formation, as neural crest cells depend on efficient ribosome production to sustain rapid proliferation.

Pathologically, haploinsufficiency or mutations in TCOF1 cause Treacher Collins syndrome (TCS), a congenital craniofacial disorder characterized by hypoplasia of facial bones and cleft palate. Defective TCOF1 disrupts ribosome biogenesis, triggering nucleolar stress and apoptosis in neural crest progenitors. Research using TCOF1 antibody supports studies in ribosome assembly, developmental biology, and congenital disease mechanisms.

TCOF1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect nucleolar proteins involved in ribosome production. NSJ Bioreagents provides TCOF1 antibody reagents optimized for cell biology, developmental genetics, and RNA metabolism research.

Structurally, Treacle protein contains multiple serine-rich and acidic domains that mediate nucleolar localization and protein-protein interactions. This antibody enables analysis of TCOF1's regulatory role in ribosome synthesis and neural crest development.

UniProt: Q13428