SPA17 anticorps (AA 1-136)

N° du produit ABIN7868130

L’anticorps anti-SPA17 Polyclonal Lapin est utilisé pour la détection de SPA17 dans des échantillons de Humain, Rat et Souris. Il a été validé pour WB, IHC, ELISA et IF.

Aperçu rapide pour SPA17 anticorps (AA 1-136) (ABIN7868130)

Antigène: SPA17 (Sperm Autoantigenic Protein 17 (SPA17))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SPA17 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Détail du produit anti-SPA17 anticorps

Épitope: AA 1-136

Fonction: SPA17 Antibody / Sperm autoantigenic protein 17

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human SPA17 recombinant protein (Position: M1-K136) was used as the immunogen for the SPA17 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the SPA17 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the SPA17 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur SPA17

Antigène: SPA17 (Sperm Autoantigenic Protein 17 (SPA17))

Autre désignation: SPA17

Sujet: SPA17 antibody recognizes Sperm autoantigenic protein 17, a testis-enriched protein encoded by the SPA17 gene that is involved in sperm motility, fertilization, and cell adhesion. SPA17 is expressed primarily in the acrosomal region of mature spermatozoa and in ciliated epithelial tissues, where it contributes to cell-cell interaction and immune recognition processes. The protein contains an N-terminal sperm-coating antigen domain and a C-terminal region homologous to the heat shock protein family, suggesting a chaperone-like role in maintaining sperm surface protein stability.

SPA17 was initially identified as an autoantigen in patients with infertility and autoimmune disorders, reflecting its immunogenic nature. It is also classified as a cancer-testis antigen due to its restricted normal expression in testicular tissue and aberrant expression in various malignancies, including ovarian, prostate, and lung cancers. This re-expression in tumors has made SPA17 a target of interest for cancer immunotherapy and tumor biomarker research. Functionally, SPA17 promotes sperm-egg interaction by binding to carbohydrate residues on the zona pellucida and may participate in intracellular signaling during acrosomal exocytosis.

The SPA17 gene is located on chromosome 11q24.2 and encodes a 151-amino acid protein that forms homodimers. The protein associates with the outer acrosomal membrane and flagellar structures, supporting motility and fertilization capacity. Beyond reproduction, SPA17 has been detected in immune and neural tissues, where it may modulate cAMP-mediated signaling pathways. Studies have shown SPA17 interaction with protein kinase A (PKA) regulatory subunits, linking it to cellular adhesion and signal transduction.

Immunohistochemical analysis using SPA17 antibody reveals strong staining in testis, epididymis, and certain tumor tissues. The antibody is widely used to investigate sperm biology, fertility disorders, and tumor immunogenicity. SPA17 antibody from NSJ Bioreagents provides a reliable reagent for studying reproductive protein function and cancer-testis antigen expression in diverse research applications

UniProt: Q15506