P57 (AA 1-200) anticorps

N° du produit ABIN7868693

Cet anticorps Souris Monoclonal détecte spécifiquement P57 dans IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour P57 (AA 1-200) anticorps (ABIN7868693)

Antigène: P57

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: KIP2-7639

Détail du produit

Épitope: AA 1-200

Fonction: p57 Antibody / p57Kip2

Purification: Protein A/G affinity

Immunogène: A recombinant fragment (within amino acids 1-200) of human p57Kip2 protein was used as the immunogen for the p57 antibody.

Isotype: IgG2b, kappa

Information d'application

Indications d'application: Optimal dilution of the p57 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the p57 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: P57

Sujet: Recognizes a protein of 57 kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.

UniProt: P49918