HDHD1 anticorps (AA 1-228)

N° du produit ABIN7868873

Cet anticorps Lapin Polyclonal détecte spécifiquement HDHD1 dans WB et ELISA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour HDHD1 anticorps (AA 1-228) (ABIN7868873)

Antigène: HDHD1 (Haloacid Dehalogenase-Like Hydrolase Domain Containing 1 (HDHD1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HDHD1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-HDHD1 anticorps

Épitope: AA 1-228

Fonction: PUDP Antibody / Pseudouridine 5-phosphatase

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human PUDP recombinant protein (Position: M1-E228) was used as the immunogen for the PUDP antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the PUDP antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the PUDP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur HDHD1

Antigène: HDHD1 (Haloacid Dehalogenase-Like Hydrolase Domain Containing 1 (HDHD1))

Autre désignation: PUDP

Sujet: PUDP antibody detects Pseudouridine 5'-phosphatase, an enzyme involved in nucleotide metabolism and RNA modification recycling. Encoded by the PUDP gene on chromosome 10q23.31, this enzyme catalyzes the dephosphorylation of pseudouridine 5'-phosphate, a modified nucleoside derived from RNA turnover. By recycling pseudouridine into usable intermediates, PUDP contributes to nucleotide homeostasis and RNA degradation pathways essential for cellular metabolism.

PUDP is a member of the haloacid dehalogenase (HAD) superfamily of hydrolases and shares structural features with sugar phosphatases, including a conserved catalytic Asp residue that facilitates phosphate hydrolysis. The enzyme functions primarily in the cytoplasm and exhibits substrate specificity for pseudouridine monophosphate over canonical nucleotides. PUDP activity supports RNA salvage by clearing modified nucleosides generated from rRNA and tRNA decay.

The PUDP antibody is used in enzymology, RNA metabolism, and nucleotide biochemistry research to study RNA turnover and modified nucleotide processing. Western blot analysis identifies a 31 kilodalton band corresponding to PUDP, while immunofluorescence shows diffuse cytoplasmic localization consistent with its metabolic role. This antibody provides a useful reagent for monitoring nucleotide recycling and evaluating enzymatic regulation in metabolic and stress-response pathways.

Altered expression of PUDP has been associated with cancer, where metabolic reprogramming changes nucleotide flux and RNA stability. By participating in modified nucleotide clearance, PUDP helps maintain nucleotide pool balance and prevents accumulation of potentially toxic intermediates. The PUDP antibody supports studies into RNA degradation, nucleotide recycling, and metabolic adaptation. NSJ Bioreagents validates this antibody for its applications ensuring specificity and reproducibility for nucleotide metabolism research.

UniProt: Q08623