EGR2 anticorps (AA 1-257)

N° du produit ABIN7868988

L’anticorps anti-EGR2 Polyclonal Lapin est utilisé pour la détection de EGR2 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, ELISA et FACS.

Aperçu rapide pour EGR2 anticorps (AA 1-257) (ABIN7868988)

Antigène: EGR2 (Early Growth Response 2 (EGR2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EGR2 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-EGR2 anticorps

Épitope: AA 1-257

Fonction: EGR2 Antibody / Early growth response protein 2

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human EGR2 recombinant protein (Position: M1-R257) was used as the immunogen for the EGR2 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the EGR2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the EGR2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur EGR2

Antigène: EGR2 (Early Growth Response 2 (EGR2))

Autre désignation: EGR2

Sujet: EGR2 antibody detects Early growth response protein 2 (also known as Krox-20), a zinc finger transcription factor that governs peripheral nerve myelination, hindbrain segmentation, and immune regulation. Encoded by the EGR2 gene on chromosome 10q21.3, this transcription factor belongs to the early growth response family of immediate-early genes activated by mitogenic and stress stimuli. EGR2 contains three C2H2-type zinc finger motifs that bind GC-rich DNA sequences to regulate genes controlling differentiation and growth arrest. It is essential for Schwann cell maturation and myelin gene expression in the peripheral nervous system.

During development, EGR2 plays a critical role in establishing segmental identity within the hindbrain by defining rhombomere boundaries and activating patterning genes. In Schwann cells, EGR2 drives transcription of myelin structural proteins including MPZ, PMP22, and MBP, ensuring proper myelin sheath formation and maintenance. Mutations in EGR2 cause hereditary neuropathies such as Charcot-Marie-Tooth disease type 1D and Dejerine-Sottas syndrome, underscoring its indispensable function in myelination. The protein also participates in T-cell anergy and macrophage activation by regulating cytokine gene expression.

The EGR2 antibody is widely used in neurobiology, developmental biology, and immunology to detect EGR2 protein expression and localization. Western blot analysis typically shows a 50 kilodalton band, while immunohistochemistry reveals strong nuclear staining in Schwann cells and developing neurons. Expression of EGR2 is inducible by growth factors, nerve injury, and mechanical stress, making it a sensitive marker for differentiation and regeneration. In the immune system, EGR2 acts as a transcriptional repressor in T cells to maintain peripheral tolerance by suppressing activation genes.

Beyond its physiological roles, EGR2 has been implicated in tumor biology and inflammatory disease. Altered EGR2 signaling can disrupt differentiation programs and contribute to leukemogenesis or autoimmune disorders. In cancer models, EGR2 functions context-dependently as either a tumor suppressor or pro-survival factor, depending on cellular environment. The EGR2 antibody allows researchers to explore these regulatory networks, providing a means to monitor transcriptional changes during neural development and immune modulation. NSJ Bioreagents provides this antibody validated for its applications, ensuring consistent and specific detection across experimental systems.

UniProt: P11161