NOXO1 anticorps (AA 1-294)

N° du produit ABIN7869065

Cet anticorps Lapin Polyclonal détecte spécifiquement NOXO1 dans WB, ELISA et IHC. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour NOXO1 anticorps (AA 1-294) (ABIN7869065)

Antigène: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NOXO1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-NOXO1 anticorps

Épitope: AA 1-294

Fonction: NOXO1 Antibody / NADPH oxidase organizer 1

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human NOXO1 recombinant protein (Position: M1-E294) was used as the immunogen for the NOXO1 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the NOXO1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the NOXO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur NOXO1

Antigène: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Autre désignation: NOXO1

Sujet: NOXO1 antibody detects NADPH oxidase organizer 1, a cytosolic adaptor protein that regulates reactive oxygen species (ROS) production by the NADPH oxidase complex. The UniProt recommended name is NADPH oxidase organizer 1 (NOXO1). This protein is a functional homolog of p47phox and is required for assembling and activating NADPH oxidase in nonphagocytic cells.

Functionally, NOXO1 antibody identifies a 376-amino-acid protein containing PX (phox homology) and SH3 (Src homology 3) domains that mediate membrane localization and protein-protein interactions. NOXO1 binds to membrane subunits such as NOX1, NOXA1, and p22phox, organizing them into an active complex that transfers electrons from NADPH to oxygen, generating superoxide. This process supports cellular signaling, host defense, and redox homeostasis.

The NOXO1 gene is located on chromosome 16q22.1 and encodes a regulatory component expressed in epithelial cells of the colon, prostate, and vascular tissues. Unlike its phagocytic counterpart p47phox, NOXO1 lacks an autoinhibitory region, resulting in constitutive activity under resting conditions. Its expression enables sustained ROS generation in nonimmune cells for physiological signaling functions such as cell proliferation and differentiation.

In redox signaling, NOXO1-derived ROS modulate kinase activation, ion transport, and gene expression. In the intestine, NOXO1 contributes to mucosal defense and microbial balance by supporting NOX1-mediated oxidative activity. In vascular and neural tissues, it influences smooth muscle tone and neuronal excitability. Dysregulation of NOXO1 expression or complex assembly has been implicated in oxidative stress-related disorders, including inflammatory bowel disease and hypertension.

NOXO1 antibody is widely used in redox biology, signaling, and oxidative stress research. It is suitable for western blotting, immunofluorescence, and co-immunoprecipitation to analyze NOXO1 expression and complex formation. This antibody aids in studying NADPH oxidase regulation, reactive oxygen generation, and oxidative signaling pathways. It is also useful in exploring NOX1 and NOX3 complex activity in epithelial and neuronal systems.

Structurally, NOXO1 features an N-terminal PX domain that binds phosphoinositides and C-terminal SH3 motifs that engage proline-rich regions of target proteins. Post-translational regulation includes phosphorylation and redox-dependent modifications that modulate its interaction with NOXA1 and NOX1. NSJ Bioreagents provides NOXO1 antibody reagents validated for use in redox signaling, ROS metabolism, and membrane complex assembly studies.

UniProt: Q8NFA2