QRSL1 anticorps (AA 1-528)

N° du produit ABIN7869453

L’anticorps anti-QRSL1 Polyclonal Lapin est utilisé pour la détection de QRSL1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, ELISA, IHC et FACS.

Aperçu rapide pour QRSL1 anticorps (AA 1-528) (ABIN7869453)

Antigène: QRSL1 (Glutaminyl-tRNA Synthase (Glutamine-Hydrolyzing)-Like 1 (QRSL1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp QRSL1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-QRSL1 anticorps

Épitope: AA 1-528

Fonction: QRSL1 Antibody / Glutamyl-tRNA amidotransferase subunit A

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human QRSL1 recombinant protein (Position: M1-Q528) was used as the immunogen for the QRSL1 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the QRSL1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the QRSL1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur QRSL1

Antigène: QRSL1 (Glutaminyl-tRNA Synthase (Glutamine-Hydrolyzing)-Like 1 (QRSL1))

Autre désignation: QRSL1

Sujet: QRSL1 antibody detects Glutamyl-tRNA amidotransferase subunit A, a mitochondrial enzyme involved in the synthesis of correctly charged glutaminyl-tRNA required for mitochondrial protein translation. Encoded by the QRSL1 gene on chromosome 6q15, this protein forms part of the heterotrimeric GatCAB complex (comprising QRSL1, GATB, and GATC) that catalyzes the transamidation of misacylated Glu-tRNA(Gln) into Gln-tRNA(Gln). This reaction maintains translational fidelity in mitochondria, ensuring proper expression of the mitochondrial genome.

Structurally, QRSL1 is a 477-amino-acid mitochondrial matrix protein of approximately 53 kilodaltons containing a glutaminase domain and an amidotransferase catalytic domain that cooperatively enable amide group transfer from glutamine to tRNA-bound glutamate. It interacts with the GATB and GATC subunits to form a functional enzyme complex anchored to mitochondrial ribosomes. QRSL1 is ubiquitously expressed, with high levels in energy-demanding tissues such as heart, muscle, and brain.

The QRSL1 antibody is widely used in mitochondrial biology, enzymology, and translation research to study aminoacyl-tRNA synthesis, mitochondrial translation accuracy, and metabolic regulation. Western blot analysis detects a 53 kilodalton band corresponding to QRSL1, while immunofluorescence reveals characteristic mitochondrial localization. This antibody serves as a reliable tool for evaluating mitochondrial translation machinery and enzyme complex formation.

Functionally, QRSL1 ensures accurate decoding of the mitochondrial genetic code by converting incorrectly charged tRNAs into their correct aminoacylated forms. Mutations in QRSL1 are linked to mitochondrial encephalopathy and lactic acidosis due to impaired oxidative phosphorylation and protein synthesis defects. The enzyme also contributes to overall mitochondrial proteostasis by preventing misincorporation of amino acids during translation. The QRSL1 antibody supports mechanistic studies of mitochondrial translation, respiratory chain biogenesis, and genetic disorders caused by tRNA modification defects. NSJ Bioreagents validates this antibody for its applications ensuring reliable detection in mitochondrial enzymatic studies.

UniProt: Q9H0R6