LIMS1 anticorps (AA 11-172)

N° du produit ABIN7870068

L’anticorps anti-LIMS1 Polyclonal Lapin est utilisé pour la détection de LIMS1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB et ELISA.

Aperçu rapide pour LIMS1 anticorps (AA 11-172) (ABIN7870068)

Antigène: LIMS1 (LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIMS1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-LIMS1 anticorps

Épitope: AA 11-172

Fonction: LIMS1 Antibody / LIM and senescent cell antigen-like-containing domain protein 1

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human LIMS1 recombinant protein (Position: E11-A172) was used as the immunogen for the LIMS1 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the LIMS1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the LIMS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur LIMS1

Antigène: LIMS1 (LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1))

Autre désignation: LIMS1

Sujet: LIMS1 antibody detects LIM and senescent cell antigen-like-containing domain protein 1, encoded by the LIMS1 gene on chromosome 2q12.3. LIMS1 antibody is widely applied in studies of integrin signaling, cell adhesion, and cancer biology. LIMS1, also known as PINCH1, is a LIM domain-containing adaptor protein that localizes to focal adhesions, where it connects integrins with the actin cytoskeleton and signaling pathways. It plays a central role in cell-matrix interactions and regulation of survival, proliferation, and migration.

Structurally, LIMS1 is a ~37 kDa protein composed of five LIM domains, which mediate protein-protein interactions. Through these domains, LIMS1 interacts with integrin-linked kinase (ILK) and parvin to form the IPP complex, a key signaling hub at focal adhesions. This complex links extracellular matrix adhesion with intracellular signaling cascades.

Functionally, LIMS1 stabilizes focal adhesions and promotes integrin-mediated signaling. It regulates pathways including PI3K-Akt, MAPK, and Rho GTPase signaling, influencing survival, motility, and cytoskeletal organization. Knockout models demonstrate that LIMS1 is essential for embryonic development and cell viability. Researchers use LIMS1 antibody to investigate adhesion signaling, integrin function, and tumor progression.

Clinically, LIMS1 is associated with cancer, where its overexpression promotes invasion and metastasis by enhancing integrin signaling. It also influences therapy resistance in multiple tumor types. Genetic studies have linked LIMS1 variants to immune function and kidney transplantation outcomes, suggesting roles beyond adhesion. NSJ Bioreagents supplies LIMS1 antibody for use in adhesion biology, immunology, and oncology.

Experimentally, LIMS1 antibody is applied in western blotting to detect the ~37 kDa protein, in immunofluorescence to visualize focal adhesion localization, and in immunohistochemistry to assess tumor expression. Co-immunoprecipitation with LIMS1 antibody identifies ILK, parvin, and integrin-binding partners.

UniProt: P48059