METTL17 anticorps (AA 129-429)

N° du produit ABIN7870440

L’anticorps anti-METTL17 Polyclonal Lapin est utilisé pour la détection de METTL17 dans des échantillons de Humain. Il a été validé pour WB, ELISA, ICC et IF.

Aperçu rapide pour METTL17 anticorps (AA 129-429) (ABIN7870440)

Antigène: METTL17 (Methyltransferase Like 17 (METTL17))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp METTL17 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Détail du produit anti-METTL17 anticorps

Épitope: AA 129-429

Fonction: METTL17 Antibody / Methyltransferase-like protein 17

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human METTL17 recombinant protein (Position: K129-R429) was used as the immunogen for the METTL17 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the METTL17 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the METTL17 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur METTL17

Antigène: METTL17 (Methyltransferase Like 17 (METTL17))

Autre désignation: METTL17

Sujet: METTL17 antibody detects Methyltransferase-like protein 17, a mitochondrial RNA methyltransferase that contributes to the biogenesis and function of mitochondrial ribosomes. METTL17 catalyzes methylation of specific rRNA nucleotides, a process essential for proper ribosomal assembly and translational fidelity within mitochondria. This enzyme is part of the large family of S-adenosylmethionine-dependent methyltransferases, many of which regulate gene expression and RNA stability. The METTL17 antibody is widely used to study mitochondrial gene expression, translation, and the broader methyltransferase network involved in RNA modification and energy metabolism.

METTL17 is encoded by the METTL17 gene located on human chromosome 11q24.2. The protein localizes primarily to the mitochondrial matrix, where it associates with mitochondrial ribosomal subunits. Structurally, METTL17 contains a conserved Rossmann-like fold typical of class I methyltransferases, which facilitates binding to S-adenosyl-L-methionine (SAM), the universal methyl donor. Loss of METTL17 function impairs mitochondrial translation, leading to reduced oxidative phosphorylation and energy production.

The METTL17 antibody is useful in identifying both endogenous and overexpressed protein in cell lysates. Western blot typically reveals a single band around 44i 1/246 kDa corresponding to full-length METTL17. Immunofluorescence analysis demonstrates mitochondrial localization with co-staining of mitochondrial markers such as TOM20 or COX IV. Functional studies have shown that METTL17 interacts with mitochondrial ribosomal RNA and components of the large ribosomal subunit, supporting its role in rRNA modification and mitoribosome assembly.

Defects in METTL17-mediated methylation have been linked to disrupted mitochondrial respiration and altered cellular energy metabolism. In addition to its mitochondrial functions, METTL17 expression has been observed in proliferating cells and certain cancer tissues, suggesting potential links between RNA methylation and tumor metabolism. NSJ Bioreagents provides a validated METTL17 antibody for western blot, immunofluorescence, and immunoprecipitation applications, enabling detailed characterization of mitochondrial RNA processing and the effects of METTL17 on bioenergetics and translation regulation.

UniProt: Q9H7H0