ERAS anticorps (AA 13-42)

N° du produit ABIN7870475

Cet anticorps Lapin Polyclonal détecte spécifiquement ERAS dans WB, IF, IHC (p) et FACS. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour ERAS anticorps (AA 13-42) (ABIN7870475)

Antigène: ERAS (ES cell expressed Ras (ERAS))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ERAS est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Détail du produit anti-ERAS anticorps

Épitope: AA 13-42

Fonction: ERAS Antibody / GTPase ERas

Purification: Purified

Immunogène: A portion of amino acids 13-42 from the human protein was used as the immunogen for the ERAS antibody.

Isotype: Ig Fraction

Information d'application

Indications d'application: The stated application concentrations are suggested starting points. Titration of the ERAS antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the ERAS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Détails sur ERAS

Antigène: ERAS (ES cell expressed Ras (ERAS))

Autre désignation: ERAS

Sujet: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells.

UniProt: Q7Z444