SMAP2 anticorps (AA 142-429)

N° du produit ABIN7870691

Cet anticorps Lapin Polyclonal détecte spécifiquement SMAP2 dans WB, ELISA, IP et FACS. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour SMAP2 anticorps (AA 142-429) (ABIN7870691)

Antigène: SMAP2 (Small ArfGAP2 (SMAP2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMAP2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Détail du produit anti-SMAP2 anticorps

Épitope: AA 142-429

Fonction: SMAP2 Antibody / Small ArfGAP 2

Purification: Immunogen affinity purified

Immunogène: E.coli-derived human SMAP2 recombinant protein (Position: K142-K429) was used as the immunogen for the SMAP2 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the SMAP2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the SMAP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur SMAP2

Antigène: SMAP2 (Small ArfGAP2 (SMAP2))

Autre désignation: SMAP2

Sujet: SMAP2 antibody detects Small ArfGAP 2, an ADP-ribosylation factor GTPase-activating protein that regulates membrane trafficking and clathrin-mediated endocytosis. The UniProt recommended name is Small ArfGAP 2 (SMAP2). This cytosolic protein functions as a regulator of ARF family small GTPases, controlling vesicle formation and cargo sorting at the trans-Golgi network and plasma membrane.

Functionally, SMAP2 antibody identifies a 429-amino-acid protein containing an ArfGAP domain and a C-terminal clathrin-binding region. SMAP2 stimulates GTP hydrolysis on ARF1 and ARF6, facilitating vesicle uncoating and recycling. It plays a key role in synaptic vesicle turnover, receptor endocytosis, and intracellular trafficking, ensuring efficient communication between organelles.

The SMAP2 gene, located on chromosome 1p34.1, is widely expressed in brain, heart, and other tissues with active vesicular transport. In neurons, SMAP2 localizes to presynaptic terminals, where it interacts with clathrin and adaptor proteins to regulate synaptic vesicle cycling. Its activity coordinates endocytosis and exocytosis, maintaining synaptic plasticity and receptor availability.

Pathologically, aberrant SMAP2 expression or dysfunction may contribute to neurological disorders, including synaptic transmission defects and neurodevelopmental abnormalities. Its regulatory role in membrane dynamics also links it to cancer cell migration and secretion. Research with SMAP2 antibody provides insight into ARF signaling, vesicle recycling, and cellular communication mechanisms.

SMAP2 antibody is suitable for western blotting, immunocytochemistry, and immunoprecipitation to detect SMAP2 in cultured cells and tissues. NSJ Bioreagents offers validated SMAP2 antibody reagents designed for studies of ARF GTPase signaling, endocytosis, and neuronal function.

Structurally, SMAP2 contains an N-terminal ArfGAP catalytic domain that interacts with GTP-bound ARF, accelerating GTP hydrolysis, and a C-terminal region that binds clathrin and adaptor proteins. This modular structure allows SMAP2 to act as a molecular bridge between vesicle formation and signaling regulation. This antibody aids in understanding SMAP2i 1/2s contribution to intracellular trafficking and membrane remodeling.

UniProt: Q8WU79