XAF1 anticorps (AA 197-301)

N° du produit ABIN7871745

L’anticorps anti-XAF1 Polyclonal Lapin est utilisé pour la détection de XAF1 dans des échantillons de Humain. Il a été validé pour WB, ELISA, IHC (p) et FACS.

Aperçu rapide pour XAF1 anticorps (AA 197-301) (ABIN7871745)

Antigène: XAF1 (XiAP Associated Factor 1 (XAF1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp XAF1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Détail du produit anti-XAF1 anticorps

Épitope: AA 197-301

Fonction: XIAP associated factor 1 Antibody / XAF1

Purification: Antigen affinity purified

Immunogène: Recombinant human protein (amino acids Q197-S301) was used as the immunogen for the XIAP associated factor 1 antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the XIAP associated factor 1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Stock: 4 °C,-20 °C

Stockage commentaire: After reconstitution, the XIAP associated factor 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Détails sur XAF1

Antigène: XAF1 (XiAP Associated Factor 1 (XAF1))

Autre désignation: XIAP associated factor 1

Sujet: XAF1, also called XIAP-associated factor 1, antagonizes the anti-caspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells. By genomic sequence analysis, the XAF1 gene contains 7 exons spanning 18 kb. Southern blot analysis suggested that XAF1 is a single-copy gene. The XAF1 gene is mapped on 17p13.1. Fluorescence microscopy demonstrated nuclear expression of XAF1, in contrast to the cytoplasmic expression of XIAP. Functional analysis indicated that XAF1 reverses the XIAP-mediated inhibition of CASP3 and its protection against apoptosis. Northern blot analysis detected wide expression of 3.9-, 4.5-, 6.0-, and 7.0-kb XAF1 transcripts, with highest levels in heart and ovary and lowest levels in brain and testis. Antisense experiments determined that loss of XAF1 expression enhances resistance to apoptosis. Alteration in XAF1 and XIAP RNA expression levels may lead to increased apoptotic resistance and proliferation due to unregulated XIAP function in cancer cells.

UniProt: Q6GPH4